Device

Part:BBa_K5090007:Design

Designed by: Manuel Marques Vilar   Group: iGEM24_Stony-Brook   (2024-10-01)


Dual Regulation System for E. coli-Based Systems


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1214
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1214
    Illegal BglII site found at 1664
    Illegal XhoI site found at 1679
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1214
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added P2A near the start to minimize potential issues of a 7-amino acid scar on LacI.


Source

This part is composed of several sub-parts, listed in the sequence.

References